Canine Heartworm Test Kit CHW
Introduction
Canine Heartworm Test Kit is designed to detect the antigens of canine heartworm (Dirofilaria immitis) in canine whole blood, serum or plasma. Two antibodies in the kit specifically bind with different epitopes of the antigens. After being absorbed into the cellulose pad, the antigens of canine heartworm move and bind with gold-colloid complex of monoclonal anti-canine heartworm of the conjugate pad, forming Ab-Ag complex. This complex, then, forms Ab-Ag-Ab direct sandwich binding with the antibody of another anti-canine heartworm in the nitrocellulose membrane. The test results can appearance on Control and Test lines where the principles of immunochromatography are used.
For Cassette
1.Review “Specimen collection” instructions. Test device, patient’s samples, and controls should be brought to room temperature (20-30℃) prior to testing. Do not open pouches until ready to perform the assay.
2.Remove the test device from its protective pouch (bring the device to room temperature before opening the pouch to avoid condensation of moisture on the membrane). Label the device with patient or control number.
3.Hold the dropper vertically and transfer 3 full drops of urine (approx. 0.15ml) to the specimen well (S) of the test device, and then start the timer. Use a separate pipette and device for each sample or control.
4.Read result between 3 to 8 minutes after the addition of samples. Do not read result after 8 minutes.
How To Read ?
Positive: Only one pink band appears on test region of the Cassette.
Negative: Two pink bands appear on test region of the Cassette.
Invalid: If without colored band appears on test region, this is an indication of a possible error in performing the test. The test should be repeated using a new device.