HiScript II Reverse Transcriptase
HiScript II Reverse Transcriptase
High efficiency • Heat resistance
1. Ultra high heat resistance
The half-life of this enzyme at 50°C exceeds 240 min, and can preserve stably for a long time at 55°C, which improves the success rate of reverse transcription with complex secondary structure templates. Meanwhile, the high activity maintained at high temperature leads to higher cDNA yield.
2. Excellent reverse transcription efficiency
Multiple points are mutated on the gene of this enzyme to further strengthen the template affinity and advancement, which greatly improves the cDNA synthesis ability and detection sensitivity.
The HiScript II Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase. The half-life of HiScript II at 50℃ is > 240 min. At 55℃, the HiScript II can also be stable for a long time, which significantly benifits the transcription of RNA templates with complex secondary structures. In addition, the HiScript II has a improved template affinity and cDNA synthesis efficiency. It has a good resistance to most RT-PCR inhibitors and is suitable for long-fragment cDNA amplification (as long as 20 kb).
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