This product uses rapid immunochromatography for the detection of foot-and-mouth disease virus type O antibodies. After that sample is added into the sample adde hole of the detection card, if the foot-and-mouth disease virus o-type antibody exists in the sample, the antibody can specifically combine with the foot-and-mouth disease virus o-type antigen marked by colloidal gold to form a compound which moves along the chromatographic membrane and is capture by the protein pre-coated on the chromatographic membrane to form a wine red detection line at the position of the clam shell t. In the absence of FMDV O-antibody in the sample, no visible line was formed at the T position. In addition, a C-line was also designed in this system to verify the effectiveness of the experiment. The line shall be coloured either negative or positive, otherwise it shall be declared invalid.